Dr. Sulchek’s BioMEMS and Biomechanics Laboratory occupies approximately 1000 sq. ft. in the Petit Inst. for Bioengineering and Bioscience (IBB) at Georgia Inst. of Technology and consists of a main wet lab and adjacent cell-culture, optical microscope/dark room, and atomic force microscopy room. The laboratory is fully equipped for DNA/RNA isolation and analysis, PCR, molecular cloning, bacterial culture, chromatography, protein biochemistry, cell/tissue isolation, cell culture, and microscopy. One atomic force microscope, the MFP-3D (Asylum Research), which utilizes a closed chamber for temperature-controlled, closed loop operation. The AFM is operated in conjunction with an advanced inverted optical microscope, the Nikon TiE. We utilize a second Nikon TiE for cell tracking in brightfield and fluorescence.
The IBB hosts a variety of equipment including 2 scanning laser confocal microscopes, multiple standard fluorescent microscopes, flow cytometry, qRT-PCR. Dr. Sulchek has access to peptide/nucleotide synthesis and gene sequencing core facilities within GA Tech.
Atomic Force Microscope (AFM)
Beckman tabletop high-speed centrifuge and minicentrifuges
Nanodrop UV/Vis spectrophotometer
Mettler-Toledo standard and analytical balances
BioRad Diad thermocycler
2 Nuaire tissue culture hood and Thermo incubators
GE Healthcare AKTApurifier FPLC with Fraction collector
Nikon TiE inverted microscope with Hamamatsu CoolSnap 16-bit CCD camera equipped for epifluorescence, phase contrast, polarized light, Perfect Focus system, with environmental chamber
4C, -20C, -80C, Liquid nitrogen storage
UV Corona Treatment Bonder
2 Harvard Apparatus syringe pumps
Light Microscopes with Micromanipulator x2
Water Bath x2