August 23, 2011

Capabilities

Dr. Sulchek’s BioMEMS and Biomechanics Laboratory occupies approximately 1000 sq. ft. in the Petit Inst. for Bioengineering and Bioscience (IBB) at Georgia Inst. of Technology and consists of a main wet lab and adjacent cell-culture, optical microscope/dark room, and atomic force microscopy room. The laboratory is fully equipped for DNA/RNA isolation and analysis, PCR, molecular cloning, bacterial culture, chromatography, protein biochemistry, cell/tissue isolation, cell culture, and microscopy. One atomic force microscope, the MFP-3D (Asylum Research), which utilizes a closed chamber for temperature-controlled, closed loop operation. The AFM is operated in conjunction with an advanced inverted optical microscope, the Nikon TiE. We utilize a second Nikon TiE for cell tracking in brightfield and fluorescence.

The IBB hosts a variety of equipment including 2 scanning laser confocal microscopes, multiple standard fluorescent microscopes, flow cytometry, qRT-PCR. Dr. Sulchek has access to peptide/nucleotide synthesis and gene sequencing core facilities within GA Tech.

 

 

Major Equipment
Atomic Force Microscope (AFM)
Beckman tabletop high-speed centrifuge and minicentrifuges
Nanodrop UV/Vis spectrophotometer
Mettler-Toledo standard and analytical balances
BioRad Diad thermocycler
2 Nuaire tissue culture hood and Thermo incubators
GE Healthcare AKTApurifier FPLC with Fraction collector
Nikon TiE inverted microscope with Hamamatsu CoolSnap 16-bit CCD camera equipped for epifluorescence, phase contrast, polarized light, Perfect Focus system, with environmental chamber
4C, -20C, -80C, Liquid nitrogen storage
UV Corona Treatment Bonder
2 Harvard Apparatus syringe pumps

Inverted Microscope System

Light Microscopes with Micromanipulator x2

Biological Safety Cabinet

Water Purification Systems

Thermal Cycler

Analytical Balance

Digital Balance

Incubator (cell culture)

Centrifuge

Mini Centrifuge

Water Bath x2

pH Meter

Shaker

Hotplate & Stirrer

Desiccator with Pumps

Ultrasonic Cleaner

Vortexer